The generally used MTS-based assay is compared with a bioassay employing a water-soluble tetrazolium dye, WST-8, using NFS-60 cells at a concentration of 7×10⁵ cells/mL against 800 IU/mL of PEGylated G-CSF at 24, 48, 72, and 72 h time points to determine the efficacy of PEGylated G-CSF. Further, the optimized WST-8 dye-based assay is used to test the potency of various commercially available PEGylated G-CSF preparations. The results demonstrate enhanced sensitivity of the WST-8-based assay over the conventional MTS-based assay for determining the potency of PEGylated G-CSF using the NFS-60 cell line^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

601.48

C₂₀H₁₃N₆NaO₁₁S₂

193149-74-5

Room temperature in continental US; may vary elsewhere.

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

In Vitro: 

H₂O : 83.33 mg/mL (138.54 mM; Need ultrasonic)

DMSO : 10 mg/mL (16.63 mM; Need ultrasonic)

配制储备液

浓度溶剂体积质量1 mg5 mg10 mg1 mM1.6626 mL8.3128 mL16.6257 mL5 mM0.3325 mL1.6626 mL3.3251 mL10 mM0.1663 mL0.8313 mL1.6626 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

• 1.

  
  

  请依序添加每种溶剂： PBS

  Solubility: 100 mg/mL (166.26 mM); Clear solution; Need ultrasonic

  
  

• 2.

  
  

  请依序添加每种溶剂： 10% DMSO    40% PEG300    5% Tween-80    45% saline

  Solubility: ≥ 1 mg/mL (1.66 mM); Clear solution

  
  

• 3.

  
  

  请依序添加每种溶剂： 10% DMSO    90% (20% SBE-β-CD in saline)

  Solubility: ≥ 1 mg/mL (1.66 mM); Clear solution

  
  

• 4.

  
  

  请依序添加每种溶剂： 10% DMSO    90% corn oil

  Solubility: ≥ 1 mg/mL (1.66 mM); Clear solution