产品编号产品名称&参数 CAS号规格库存单价(¥)JP-22010 水溶性四氮唑-8 >98% 193149-74-5 50mg 2 550.00JP-22010 水溶性四氮唑-8 >98% 193149-74-5 250mg 3 1050.00JP-22010 水溶性四氮唑-8 >98% 193149-74-5 1g 3 2800.00生物活性WST-8 is a water-soluble tetrazolium dye, WST-8 enhances sensitivity of the WST-8-based assay over the conventional MTS-based assay.体外研究(In Vitro)The generally used MTS-based assay is compared with a bioassay employing a water-soluble tetrazolium dye, WST-8, using NFS-60 cells at a concentration of 7×105 cells/mL against 800 IU/mL of PEGylated G-CSF at 24, 48, 72, and 72 h time points to determine the efficacy of PEGylated G-CSF. Further, the optimized WST-8 dye-based assay is used to test the potency of various commercially available PEGylated G-CSF preparations. The results demonstrate enhanced sensitivity of the WST-8-based assay over the conventional MTS-based assay for determining the potency of PEGylated G-CSF using the NFS-60 cell line[1].MCE has not independently confirmed the accuracy of these methods. They are for reference only.分子量601.48FormulaC20H13N6NaO11S2CAS 号193149-74-5运输条件Room temperature in continental US; may vary elsewhere.储存方式4°C, sealed storage, away from moisture and light*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)溶解性数据In Vitro: H2O : 83.33 mg/mL (138.54 mM; Need ultrasonic)DMSO : 10 mg/mL (16.63 mM; Need ultrasonic)配制储备液浓度溶剂体积质量1 mg5 mg10 mg1 mM1.6626 mL8.3128 mL16.6257 mL5 mM0.3325 mL1.6626 mL3.3251 mL10 mM0.1663 mL0.8313 mL1.6626 mL*请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。In Vivo:请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶1.请依序添加每种溶剂: PBSSolubility: 100 mg/mL (166.26 mM); Clear solution; Need ultrasonic2.请依序添加每种溶剂: 10% DMSO 40% PEG300 5% Tween-80 45% salineSolubility: ≥ 1 mg/mL (1.66 mM); Clear solution3.请依序添加每种溶剂: 10% DMSO 90% (20% SBE-β-CD in saline)Solubility: ≥ 1 mg/mL (1.66 mM); Clear solution4.请依序添加每种溶剂: 10% DMSO 90% corn oilSolubility: ≥ 1 mg/mL (1.66 mM); Clear solution